true. single. molecule. sequencing.

Global Gene Expression Studies

Unbiased and accurate quantitation of global gene expression with high dynamic range, from extremely rare transcripts to high abundance housekeeping genes.

TSMS™ Biomarker Discovery

SeqLL’s tSMS service allows the detection of subtle changes in gene expression patterns that distinguish healthy and disease states with the superior resolution.

Rare Point Mutation Detection

Rare de novo mutations in cell free RNA, Cirulating Tumor Cells & FFPE samples are identified with SeqLL’s increased detection sensitivity & higher depth of coverage.

Other Applications

Transcription Start Site Identification, Splice Variant Analysis, Allele Specific Expression Studies, Non-coding RNA Discovery & Analysis, microRNA Discovery & Analysis, And More…

DRS®

SeqLL’s DRS genetic testing and mapping services is an important step forward in the RNA Sequencing world. For the first time, researchers can explore the RNA transcriptome directly as it exists In-vivo, with no conversion to cDNA or amplification required. DRS™ service captures polyadenylated RNA directly without conversion to cDNA. Sequence RNA directly from the flow cell surface.

tSMS ® FORMALIN FIXED PARAFFIN EMBEDDED (FFPE)

SeqLL’s tSMS services is uniquely suited to analyze damaged or fragmented nucleic acids from formalin fixed paraffin embedded samples and other difficult sources. Sample degradation during processing or storage can be a challenge for other NGS systems.

Micro RNA Sequencing

True Single Molecule Sequencing is ideally suited to miRNA and other short RNA species.

CUSTOM SEQUENCING, MAPPING & TESTING

Bring us your most challenging RNA sequencing projects. Our experienced team will work with you to design a custom program to generate the publication quality data you require. The unique research and development capabilities of the tSMS allow us to succeed where others have failed.

RNA - SEQ

Transcriptome studies require quantitative sequencing in order to determine levels of gene expression for a large number of transcripts in a given sample. In many sample types, abundant but uninteresting transcripts, such as ribosomal RNA or housekeeping gene transcripts can make up the majority of the sequencing reads and can necessitate greater depth of coverage to generate enough reads of lower abundance transcripts.

In addition to the standard reverse transcriptase method using random hexamers or dT primers, SeqLL offers a not-so-random (NSR) primer approach to selectively remove high abundance transcripts from the sample during the reverse transcription step of our cDNA-based RNA-Seq protocol. Taking advantage of the high tolerance of reverse transcriptase to mis-prime, it is possible to use as few as 40 pseudo-random (PS) reverse transcription primers to decrease the rate of undesirable abundant sequences within a library without affecting the overall transcriptome diversity. (1)

(1) Courtesy of Dr. Charles Plessy, RIKEN JAPAN See Source

PS primers are simple to design and can be used to deplete specific undesirable RNAs simultaneously, thus creating a flexible tool for enriching transcriptome libraries for rare transcript sequences. Let our team work with you to create a NSR primer approach specific for your application.